Molecular Variants in SIRT1 Gene Among Saudi Women Diagnosed with Gestational Diabetes Mellitus: A Case-Control Study.

PMID 40757266 | PMCID PMC12316047 | DOI 10.2147/IJWH.S510344 · International journal of women's health · 2025 · View on PubMed ↗

BACKGROUND: Gestational Diabetes Mellitus (GDM) is defined as impaired glucose intolerance resulting in hyperglycemia. SIRT1 deficiency and single nucleotide polymorphisms (SNPs) were also related to diabetes. No documented studies were carried out between GDM and SIRT1 SNPs and it is also not confirmed whether SIRT1 SNPs had a role in GDM women. OBJECTIVE: This study was designed to explore the molecular association carried out between GDM women and SIRT1 gene SNPs present in the Saudi Arabia. This is a case-control study carried out in Capital city of Saudi Arabia. METHODS/DESIGN: Serum samples were used for studying biochemical parameters, genotyping and Sanger sequencings for rs4746720 and rs10823112 SNPs. Statistical analysis was carried out between GDM and non-GDM women for anthropometric, biochemical and molecular data. RESULTS: A total of 120 GDM and 120 non-GDM women were recruited based on inclusion and exclusion criteria. Different forms of glucose values have confirmed the statistical association levels between GDM and non-GDM women (p < 0.05). Penalized logistic regression analysis showed the positive association with FBG, OGTT-2hr and HDLc levels (p < 0.05) in GDM women. The rs4746720 SNP was associated with GDM (p = 0.01). ANOVA analysis has confirmed the strong association with FBG (p = 0.002), PPBG (p = 0.0001) and HDLc (p = 0.0003) levels in rs4746720 SNP; while in rs10823112 SNP, both PPBG (p = 0.0001) and HDLc (p = 0.01) were associated. Linkage disequilibrium and GMDR analysis showed significant associations (p < 0.05). CONCLUSION: This study confirms rs4746720 SNP and glucose levels played a role in this study.